Yeast Nitrogen base without Amino acids, without Ammonium sulphate, without Copper and without Iron

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Description

Yeast Nitrogen base without Amino acids, without Ammonium sulphate, without Copper and without IronCupric sulphate, CuSO4. 5H2O, and Ferric chloride, FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium

2 at 25°C Suspend 22 gram powdered medium in 1 litre distilled water

A concentrated alkaline buffering agent maintaining stable pH in biological research

Vitamins and Agar without Amino Acids

such as filamentous growth

0 L-Serine 1051

Suspend 47

Current Protocols

Yeast extract 5 Dextrose 30 Agar 17 Total 52

Yeast extract 10 Peptone 20 Glucose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

galactose and raffinose as carbon sources

Glycerol 252

Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin

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