Yeast Nitrogen base without Amino acids and without Folic Acid

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Description

Yeast Nitrogen base without Amino acids and without Folic AcidFolic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature

5% Sulphated Ash <= 0

03 Crystal violet 0

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen

Tryptone 8 Yeast extract 5 NaCl 5 Agar 7 Final pH 7

5 (50 g/l) Assay ≥98%

Suspend 25

04 Suspend 50 gram powdered medium in 1 litre distilled water

87 Suspend 19

Hydrolysis is not completed until all the nitrogen in the casein is converted to amino acids or other compounds of relative chemical simplicity

Peptone 10 Glucose 40 Agar 20 Final pH 7

Chemical formula C3H7NO2S Solubility (20° C) 160 g/l Mol

yeast extract is not suitable for media intended for the study of sugar fermentation